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antibodies against itga7  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology antibodies against itga7
    Antibodies Against Itga7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B17+integrin/Integrin+%CE%B17+Antibody/10__3390_slash_medicina62040670-61-21-25
    Average 94 stars, based on 30 article reviews
    antibodies against itga7 - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: High levels of sarcospan are well tolerated and act as a sarcolemmal stabilizer to address skeletal muscle and pulmonary dysfunction in DMD
    Article Snippet: For antibodies raised in mouse, Mouse on Mouse blocking reagent (Vector Laboratories) was used according to manufacturer’s instructions. .. Sections were incubated in primary antibody in PBS at 4 °C overnight with the following antibodies: dystrophin (MANDYS1; 1:5; Development Studies Hybridoma Bank), utrophin (DRP, 1:100), β-DG (MANDAG2; 1:50; Development Studies Hybridoma Bank), α-SG (VP-A105; 1:30; Vector Laboratories), β-SG (VP-B206; 1:30; Vector Laboratories), α7 integrin (C-20; 1:100; Santa Cruz), nNOS (A-11, 1:250; Santa Cruz), laminin (L9393; 1:500; Sigma-Aldrich), β1D integrin (MAB1900; 1:20; Millipore), and SSPN (E2; 1:100). .. Primary antibodies were detected by biotinylated anti–rabbit (BA-1000; 1:500; Vector Laboratories) and biotinylated anti–mouse (BA-9200; 1:500; Vector Laboratories).

    Article Title: The effect of hypoxia on myogenic differentiation and multipotency of the skeletal muscle-derived stem cells in mice
    Article Snippet: .. Briefly, 10 μL of goat anti mouse IgG magnetic bead solution was incubated with 5–10 μg of mouse CD90, CD44 (1:50, DSHB) and α7 integrin (1:50, Santa Cruz) primary antibodies in 5% FCS/PBS with at 4 °C for 1 h. After three successive washing in PBS, 1 × 10 7 cells were incubated with the antibodies beads mixture at 4 °C for 30 min. ..

    Article Title: Characterisation of stemness and multipotency of ovine muscle‐derived stem cells from various muscle sources
    Article Snippet: .. The cells were then incubated with primary antibodies, including anti‐mouse Pax7 and anti‐mouse Myogenin (1:30, DSHB, University of Iowa), α7‐integrin (1:50, Santa Cruz Biotechnology), anti‐mouse MyoD (1:100, BD Biosciences), and anti‐mouse CD90 (1:50, Antibody‐online GmbH), diluted in blocking buffer at 4°C overnight. .. The primary antibody was detected using Cy3 goat anti‐mouse secondary antibody (Dianova), diluted 1:100 in blocking buffer after incubation for 1 h in the dark at RT.

    Blocking Assay:

    Article Title: Characterisation of stemness and multipotency of ovine muscle‐derived stem cells from various muscle sources
    Article Snippet: .. The cells were then incubated with primary antibodies, including anti‐mouse Pax7 and anti‐mouse Myogenin (1:30, DSHB, University of Iowa), α7‐integrin (1:50, Santa Cruz Biotechnology), anti‐mouse MyoD (1:100, BD Biosciences), and anti‐mouse CD90 (1:50, Antibody‐online GmbH), diluted in blocking buffer at 4°C overnight. .. The primary antibody was detected using Cy3 goat anti‐mouse secondary antibody (Dianova), diluted 1:100 in blocking buffer after incubation for 1 h in the dark at RT.

    Cell Culture:

    Article Title: Nogo-A regulates myogenesis via interacting with Filamin-C
    Article Snippet: C2C12 myoblast cells, an immortalized mouse myoblast cell line, were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Corning, #10-013-CUR) supplemented with 10% fetal bovine serum (Corning, #35-015-CU) and 100 U/mL penicillin–streptomycin (Gibo-BRL, #15140122) in a humidified atmosphere containing 5% CO 2 at 37 °C. .. To induce myotube differentiation, cells were washed with PBS and cultured with DM (DMEM containing 2% horse serum [Gibco-BRL, #16050-122] and 100 U/mL penicillin–streptomycin). iMSCs were developed from mouse embryonic fibroblasts and sort-iMSCs were obtained by sorting iMSCs with stem cell markers including CD106 (Pharmingen, #01812D) and α7 integrin (Santa Cruz, sc-50431), as previously described . .. Muscle tissues were fixed in 4% paraformaldehyde (Sigma) in PBS for 24 h and immersed in gradient sucrose solutions (30% and 35%) in PBS for 6 and 16 h, respectively.

    other:

    Article Title: Syndecan-1 Regulates Cell Migration and Fibronectin Fibril Assembly
    Article Snippet: α7 integrin , Santa Cruz Biotech, Santa Cruz, CA , sc-27710 , IB.

    Isolation:

    Article Title: Thrombospondin expression in myofibers stabilizes muscle membranes
    Article Snippet: Two days later, cardiomyocyte lysates were harvested and immunoprecipitated with anti-Flag magnetic beads (Sigma-Aldrich, M8823). .. Immunoprecipitates were resolved by SDS-PAGE, transferred to PVDF membranes, and immunoblotted for Flag (Cell Signaling Technology, 2368; 1:1000); β1D-integrin (Millipore, MAB1900, 1:500); α7-Integrin (Santa Cruz Biotechnology, sc-27706; 1:200); and β-dystroglycan (Development Studies Hybridoma Bank, MANDAG2 clone 7D11; 1:100). β1D-integrin positive intracellular vesicles were isolated from quadriceps using an endoplasmic reticulum isolation kit (Sigma Aldrich, ER0100), according to the manufacturer’s instructions. ..



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